HMS Ocean is currently moored at the South Mole, overlooking Gibraltar.
On Sunday Chinook helicopters arrived in Gibraltar packed with supplies, ready to be loaded onto the ship.
HMS Ocean will be joining RFA Mounts Bay, which is already operating in the region.
The Royal Fleet Auxiliary ship has already delivered six tonnes of emergency aid to Anguilla, a British Overseas Territory devastated by last week's hurricane, and has since arrived in the British Virgin Islands to provide further support.
HMS Ocean was operating in the Mediterranean but was re-tasked last week to support the humanitarian aid and disaster relief efforts.
She has six helicopter operating spots on her flight deck with space in the hangar for many more.
Ocean currently has around 630 Royal Navy, Royal Marine and Army personnel embarked and can carry a significant amount of aid to the affected areas. She's due to depart tomorrow.
Last month, HMS Ocean departed Plymouth to begin a nine-month deployment to the Mediterranean as the flagship for NATO’s Standing Maritime Group 2, before she is decommissioned next year.