The MV22 Osprey based at RAF Midenhall in Suffolk, landed on Mounts Bay whilst she took part in the Littoral Strike Group (North) deployment.
The iconic Osprey takes off and lands just like a helicopter, then rotates its propellers to fly like a conventional aircraft.
Flight Deck Officer Mick Burton was among the team who made the aircraft landing possible.
He said: "After lots of preparation for today, myself and the team were delighted to work with such a fascinating aircraft and I look forward to working with it lots more in the coming weeks."
The Osprey’s debut on Mounts Bay is the result of several months of planning and work, including preparation work in Portland at the beginning of April, the Navy said.
The milestone has added significance as one of the RFA's Bay-class ships is lined up for conversion into a Littoral Strike Ship, meaning it will be central to future commando operations and will continue to work with the US military.
Operated by the US Marine Corps and US Air Force, the Osprey is the main aircraft for carrying American marines and related units into battle.
It can fly higher, further and faster than a helicopter and thanks to its tiltrotor design, can land in and take off from tight spots.
Cover image: RFA Mounts Bay, the first Royal Fleet Auxiliary vessel to operate with the US military’s Osprey aircraft (Picture: Royal Navy).