HMS Defender received fuel from USS America in the Philippine Sea, making it the first time a British warship has conducted an RAS with a US Navy amphibious assault ship.
Earlier in the week, HMS Defender carried out an RAS with RFA Fort Victoria, at the same time as conducting flying operations.
Carrier Strike Group Commander Commodore Steve Moorhouse posted a timelapse of Defender's live munitions replenishment by RFA Fort Victoria on Twitter.
It showed the first live munitions replenishment at sea to a Queen Elizabeth Class carrier from a solid support ship, with 14,540Kg of munitions transferred.
This was all part of the sustainment of the UK's Carrier Strike Group 21 (CSG21) at sea.
On Wednesday, HMS Kent also conducted a replenishment at sea (RAS) with RFA Fort Victoria while on operations in the Indo-Pacific region.
The refuelling took place in the morning at the same time as USS The Sullivans, part of the Carrier Task Group, conducted routine operations and exercises in the Philippine Sea.